2 years ago
Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?
Experiment: DNA isolation / purification Tissue - liver
Firstly, you should try to macerate the sample as much as you can. Then you should incubate it in proteinase K for an extended amount of time of approximately 12 hours. Finally, for the elution, warm up the buffer to 60 degree C, use only half of the recommended amount of buffer and let the solution sit on the membrane for 10 minutes.