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2 years ago

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

Experiment: DNA isolation / purification Yeast - Cryptococcus neoformans

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I would suggest that you run a real-time PCR assay with mtDNA and nuclear DNA primer sets. By doing that you will enrich the mtDNA/nDNA and you will be able to verify the purity of your preparation. Then you can run the extracted DNA on an agarose gel and extract only the mtDNA band(s).

Answered 2 years ago


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