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1 year ago

Reactivate dormant Lactobacillus Paracasei

Hello fellow Labettors, I would like to know what would be the best method/medium to reactivate my dormant Lactobacillus paracasei cells?. Any suggestions are greatly appreciated.

Experiment:

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Hello Janardhan, You should be able to do that by inoculating a colony in MRS broth and then incubating it for a week at 37 °C. Check the tube every day and if you see some turbidity subculture it 3-4 times in order to get an active culture.

Answered 1 year ago


2 Comments


Try this: Incubate MRS broth anaerobically at least overnight. Inoculate with as large an inoculum of L. paracasei as you can spare. Incubate anaerobically at between 30 to 37 °C until you observe inreased turbidity. Passage similarly two or three more times. Then transfer to agar plates.

Answered 11 months ago


Hello Janardhan, You should be able to do that by inoculating a colony in MRS broth and then incubating it for a week at 37 °C. Check the tube every day and if you see some turbidity subculture it 3-4 times in order to get an active culture.

Answered 1 year ago


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