2 years ago
During centrifugation, the column got clogged and I was unable to continue with the protocol. How can I unclog it?
Experiment: DNA isolation / purification Cells - primary cells bone marrow mononuclear cells
Try not to exceed the recommended volume of starting material that is listed by the manufacturer and if this still occurs try to use an even smaller volume. You can try to fix the clogged one by increasing the g force and centrifuging the sample for a longer period of time. Also, make sure that the columns actually work. There is always the chance that you got a faulty batch and you should contact the manufacturer about it.
Answered 2 years ago
Paul G. Macon
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