1 year ago
Hello, can someone here help me? I am trying to silence e-selectin and ICAM-1 in endothelial cells. I would like to know if this is possible using shRNA
Experiment: shRNA gene silencing Mouse - Prostate cancer cell lines (DU145 and PC3) CD24 lentiviral particles
Hello Burton, Generally it should be possible to target multiple genes however all of your constructs will contain the same selection marker. As a result, it would be impossible to determine which cells within your culture got both of the virus types.
Answered 1 year ago
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