ChIP H3K27me3 Human Rat

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - RCC

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Human - PBMC

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Human - HUVEC

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Human - HeLa

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - SMMC-7721

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Human - THP-1

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - HEK 293

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - SH-SY5Y

Get tips on using Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody to perform Western blotting AKT