Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Get tips on using Anti-TAU antibody produced in rabbit to perform Western blotting Tau
Get tips on using MMP13 Recombinant Rabbit Monoclonal Antibody (3H13L17) to perform Western blotting MMP-13
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Get tips on using ICAM-1 Recombinant Rabbit Monoclonal Antibody (9H21L19) to perform Western blotting ICAM-1
Get tips on using Cyclin D1 Recombinant Rabbit Monoclonal Antibody (SP4) to perform Western blotting Cyclin D1
Get tips on using Anti-β2-Microglobulin antibody produced in rabbit to perform Western blotting β₂ microglobulin
Get tips on using Anti-AP-1 antibody produced in rabbit to perform Western blotting C-Jun
Get tips on using Phospho-FAK (Tyr397) Recombinant Rabbit Monoclonal Antibody (31H5L17) to perform Western blotting Focal adhesion Kinase (FAK)
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