Protein isolation Yeast

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

Get tips on using E.Z.N.A.®Yeast Plasmid Mini Kit to perform Plasmid Isolation S. cerevisiae

Get tips on using Yeast Extract to perform Bacterial cell culture media Legionella pneumophilia

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using CelLytic™ Y Plus Kit to perform Protein isolation Yeast - Candida boidinii

Get tips on using CelLytic™ Y Plus Kit to perform Protein isolation Yeast - Saccharomyces cerevisiae

Get tips on using CelLytic™ Y Plus Kit to perform Protein isolation Yeast - Pichia pastoris

Get tips on using LIVE/DEAD™ Yeast Viability Kit to perform Live / Dead assay yeast - Urediniospore