Get tips on using InVivoMAb anti-mouse CD16/CD32 to perform Flow cytometry Anti-bodies Mouse - CD16/CD32
Get tips on using Purified anti-mouse CD68 Antibody to perform Flow cytometry Anti-bodies Mouse - CD68
Get tips on using APC anti-mouse CD68 Antibody to perform Flow cytometry Anti-bodies Mouse - CD68
Get tips on using PE anti-mouse CD69 Antibody to perform Flow cytometry Anti-bodies Mouse - CD69
ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
Get tips on using MUC2 (MRQ-18) Mouse Monoclonal Antibody to perform Immunohistochemistry Human - Muc-2
Get tips on using PE anti-human CD52 Antibody to perform Flow cytometry Anti-bodies Human - CD52
Get tips on using PE anti-human CD51 Antibody to perform Flow cytometry Anti-bodies Human - CD51
Get tips on using Human ICAM1 ELISA Kit (CD54) (ab100640) to perform ELISA Human - ICAM-1/CD54
Get tips on using Human PLZF Antibody to perform Immunohistochemistry Mouse - PLZF
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