Protein expression and purification Bacteria Origami™ B (DE3)

Get tips on using pCMV-HA2/DKK1 to perform Protein Expression Eukaryotic cells - HEK293 DKK1

Get tips on using pgMAX system-DsRed2 to perform Protein Expression Eukaryotic cells - HEK293 DsRed2

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Bronchi

Get tips on using PureZOL™ RNA Isolation Reagent to perform RNA isolation / purification Tissue - Rat Bone

Get tips on using pNIC28‐Bsa4-bops to perform Protein Expression Prokaryotic cells - E. coli BRs

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - HepG2 FHIT

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Hep3B SFRP3

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Get tips on using pET28a+-BL1 to perform Protein Expression Prokaryotic cells - E. coli DsbA BL1

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Human aortic endothelial cells