ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α

Get tips on using Human Decorin ELISA Kit to perform ELISA Human - Decorin

Get tips on using VWF (Human) ELISA Kit to perform ELISA Mouse - vWF-A2

Get tips on using Rat Transferrin ELISA Kit (ab137993) to perform ELISA Rat - Transferrin (Tf)

Get tips on using Rat Transferrin ELISA Kit (Colorimetric) to perform ELISA Rat - Transferrin (Tf)

Get tips on using Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides to perform 3D Cell Culture Media hiPSC-derived cardiac organoids

Get tips on using Mouse KIM-1 ELISA Kit to perform ELISA Mouse - KIM-1

Get tips on using KIM-1 (human) ELISA kit to perform ELISA Human - KIM-1

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Mouse Adiponectin ELISA Kit (ab108785) to perform ELISA Mouse - Adiponectin

Get tips on using Rat Adiponectin ELISA Kit (ab108784) to perform ELISA Rat - Adiponectin