Immunohistochemistry Anti-mouse IgG Donkey

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD49f/ITGA6

Get tips on using CD14 Antibody PE-labelled to perform FACS CD14 - Mouse Human PE

Get tips on using ATG5 Antibody Novus to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Mouse liver

Get tips on using Mre11 Antibody #4895 to perform ChIP Anti-bodies MRE11

Get tips on using LC3B monoclonal antibody (2G6) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using ATG5 Antibody (N-term) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Mouse embryonic fibroblasts

Get tips on using CtIP antibody (mAb) to perform ChIP Anti-bodies CtIP/BCL11A