DNA methylation profiling Gene specific profiling SiHa

Get tips on using CpG Methyltransferase (M.SssI) to perform PCR Methylation specific PCR - Bacterial DNA

Get tips on using EpiTect MSP Kit to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using m6a rna methylation quantification kit to perform RNA quantification Coloremetric

Get tips on using EpiTect MethyLight PCR +ROX Vial Kit (200) to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using RHOC siRNA(h) to perform siRNA / miRNA gene silencing Human - SiHa RHOC

Get tips on using MCM4 shRNA (h) Lentiviral Particles to perform shRNA gene silencing Human - SiHa MCM4

Get tips on using pSilencer™ 4.1-CMV neo to perform shRNA gene silencing Human - SiHa AEG-1

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized SiHa

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.