Immunohistochemistry Wilms Tumor 1 (WT1) Rabbit Mouse

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - THP 1

Get tips on using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Mouse - IGF-I

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Thyroid Transcription Factor-1 to perform Immunohistochemistry Human - TTF-1

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit to perform ELISA Rat - TGF-beta 1

Get tips on using LC3B (D11) XP® Rabbit mAb (Biotinylated) to perform Autophagy assay cell type - rMC-1

Get tips on using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Rat - IGF-I