Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Spathaspora passalidarum
Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Yarrowia lipolytica
Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Candida sonorensis
Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Candida albicans
Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Saccharomyces cerevisiae
Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Pichia pastoris
Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Scheffersomyces (Pichia) stipitis
Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Bacteria - Escherichia coli
Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.
Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.
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