ChIP H3K27me3 Human Mouse

Get tips on using BV421 Mouse Anti-Human CD27 to perform Flow cytometry Anti-bodies Human - CD27

Get tips on using PE Mouse anti-Human CD105 to perform Flow cytometry Anti-bodies Human - CD105

Get tips on using FITC Mouse Anti-Human CD71 to perform Flow cytometry Anti-bodies Human - CD71

Get tips on using APC Mouse Anti-Human CD71 to perform Flow cytometry Anti-bodies Human - CD71

Get tips on using FITC Mouse Anti-Human CD90 to perform Flow cytometry Anti-bodies Human - CD90

Get tips on using PE Mouse Anti-Human CD90 to perform Flow cytometry Anti-bodies Human - CD90

Get tips on using BV605 Mouse Anti-Human CD15 to perform Flow cytometry Anti-bodies Human - CD15

Get tips on using PE Mouse Anti-Human CD44 to perform Flow cytometry Anti-bodies Human - CD44

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Human - Glioblastoma cell line