Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Mouse - Gonadotrope cell lines
Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - 3T3-L1 cells
Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Mouse - 3T3-L1 cells
Get tips on using High-Sensitivity ChIP Kit (ab185913) to perform ChIP Mouse - MLL-AF9/NrasG12D AML
In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.
Get tips on using Anti-Mre11 antibody - ChIP Grade (ab12159) to perform ChIP Anti-bodies MRE11
Get tips on using Anti-CTCF antibody - ChIP Grade (ab70303) to perform ChIP Anti-bodies CTCF
Get tips on using Anti-Sall4 antibody - ChIP Grade (ab29112) to perform ChIP Anti-bodies Sall4
Get tips on using Anti-HDAC1 antibody - ChIP Grade (ab7028) to perform ChIP Anti-bodies HDAC1
Get tips on using Magna ChIP™ G Tissue Kit to perform ChIP Mouse - Cardiac fibroblasts
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