crispr-mouse-deletion-c2c12-dmd

Get tips on using pLX-sgRNA to perform CRISPR Human - Deletion CIITA

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion AXL

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion NGRN

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion SST3B

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion SST3A

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion EGFP1

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion TLN2

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion SLX4

Get tips on using lentiCRISPR v2 to perform CRISPR Rat - Deletion PC12 MMP9

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.