Protein Expression Eukaryotic cells T. reesei

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RNA isolation / purification Cells - primary mouse cortical neurons Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary mouse cortical neurons

RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus Experiment

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus aureus

PhosphoThreonine Antibody Q7 (100 µg) Product

Get tips on using PhosphoThreonine Antibody Q7 (100 µg) to perform Protein tag Detection of proteins containing phosphorylated threonine residues

Products Qiagen PhosphoThreonine Antibody Q7 (100 µg)

Senescence Cells Histochemical Staining Kit Product

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - CHO