shRNA gene silencing Mouse P19

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - mouse cardiac fibroblasts

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - mouse embryonic fibroblasts

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 7 (Dako Omnis) Clone OV-TL 12/30 to perform Immunohistochemistry Human - CK7

Get tips on using DIA-310: Anti-CD31 (Ms) from Rat (Clone: SZ31) for mouse FFPE tissue to perform Immunohistochemistry CD31 - Rabbit Rat -NA-

Get tips on using Imprint® Methylated DNA Quantification Kit to perform DNA methylation profiling Gene specific profiling - Mouse muscle stem cells SPRY1

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - mouse pancreatic stellate cells

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.