ChIP H3K27me3 Rat Rabbit

Get tips on using PCNA (D3H8P) XP® Rabbit mAb #13110 to perform Immunohistochemistry Mouse - PCNA

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Get tips on using Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147 to perform Immunohistochemistry Mouse - Notch1

Get tips on using VEGF Receptor 2 (55B11) Rabbit mAb #2479 to perform Western blotting VEGF

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using ICAM-1 Recombinant Rabbit Monoclonal Antibody (9H21L19) to perform Western blotting ICAM-1

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Get tips on using DIA-310: Anti-CD31 (Ms) from Rat (Clone: SZ31) for mouse FFPE tissue to perform Immunohistochemistry CD31 - Rabbit Rat -NA-