Flowcytometry CD14 Mouse / IgG2a

Get tips on using Mouse Dkk-1 Quantikine ELISA Kit to perform ELISA Mouse - Dkk-1

Get tips on using ScriptSeq Complete Kit (Human/Mouse/Rat) to perform RNA sequencing Mouse - J774

Get tips on using APC anti-mouse CD206 (MMR) Antibody to perform Flow cytometry Anti-bodies Mouse - CD206

Get tips on using Mouse CD200 R3 PE-conjugated Antibody to perform Flow cytometry Anti-bodies Mouse - CD200

Get tips on using Mouse PAI1 ELISA Kit (SERPINE1) (ab197752) to perform ELISA Mouse - Serpin E1/PAI-1

Get tips on using ANGPTL3 (mouse/rat) Dual ELISA Kit to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using NeuroCult™ Basal Medium (Mouse & Rat) to perform 3D Cell Culture Media Mouse embryonic neurospheres

Get tips on using Purified anti-mouse TNF-α Antibody to perform Flow cytometry Anti-bodies Mouse - TNF-α

Get tips on using FITC anti-mouse CD95 (Fas) Antibody to perform Flow cytometry Anti-bodies Mouse - CD95/Fas