Protein Expression Eukaryotic cells T. pseudonana

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ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type human umbelical vein endothelial cells (HUVEC)

Get tips on using Whole Rat Genome Microarray Kit, 4x44K to perform Microarray Gene expression arrays - Rat chorid plexus Cyanine 3

Products Agilent Technologies Whole Rat Genome Microarray Kit, 4x44K

Get tips on using GeneChip™ Rat Genome 230 2.0 Array to perform Microarray Gene expression arrays - Rat mesothelium Satin cocktail

Products Thermo Fisher Scientific GeneChip™ Rat Genome 230 2.0 Array

Get tips on using GeneChip™ Hybridization, Wash, and Stain Kit to perform Microarray Gene expression arrays - Rat mesothelium Satin cocktail

Products Thermo Fisher Scientific GeneChip™ Hybridization, Wash, and Stain Kit

Get tips on using Streptavidin-R-PE to perform Protein tag Detection of biotinylated proteins

Products Qiagen Streptavidin-R-PE

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines Lymphoblastoid cell lines

RNA siRNA / miRNA gene silencing Rat Glial cells C/EBP‐β

RNA siRNA / miRNA gene silencing Rat Retinal stem cells Brn-3b

Get tips on using Whole Mouse Genome Microarray Kit, 4x44K to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Products Agilent Technologies Whole Mouse Genome Microarray Kit, 4x44K

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse brain tissue Biotin

Products Thermo Fisher Scientific GeneChip® HT 3' IVT PLUS Reagent Kit

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