siRNA / miRNA gene silencing Human PEL

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

Get tips on using EasySep™ Direct Human B Cell Isolation Kit to perform Cell Isolation B cell

Get tips on using EasySep™ Release Human CD19 Positive Selection Kit to perform Cell Isolation B cell

Get tips on using MojoSort™ Human B Cell (CD43-) Isolation Kit to perform Cell Isolation B cell

Get tips on using Dynabeads™ Untouched™ Human B Cells Kit to perform Cell Isolation B cell

Get tips on using Complete Kit for Human Whole Blood CD34+ Cells to perform Cell Isolation CD34+ cells

Get tips on using EasySep™ Human CD34 Positive Selection Kit II to perform Cell Isolation CD34+ cells

Get tips on using Human Total HO-1/HMOX1 DuoSet IC ELISA to perform ELISA Rat - HO-1