siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

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Get tips on using hMSC Human Mesenchymal Stem Cell Chondrogenic Differentiation Basal Medium to perform Stem cell Differentiation media mPericytes differentiation into Chondrogenic cells

Products Lonza hMSC Human Mesenchymal Stem Cell Chondrogenic Differentiation Basal Medium

Get tips on using Human Thrombopoietin R/Tpo R APC-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD110/Thrombopoietin R

Products R&D Systems Human Thrombopoietin R/Tpo R APC-conjugated Antibody

Get tips on using Human Thrombopoietin R/Tpo R PE-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD110/Thrombopoietin R

Products R&D Systems Human Thrombopoietin R/Tpo R PE-conjugated Antibody

Get tips on using Human IL-3R alpha /CD123 PE-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Products R&D Systems Human IL-3R alpha /CD123 PE-conjugated Antibody

Get tips on using PE-Cy™7 Mouse Anti-Human CD123 to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Products BD Biosciences PE-Cy™7 Mouse Anti-Human CD123

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type Human melanocytes

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Cellular assays Cell cycle assay human AGS cell line

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Cellular assays Cell cycle assay human MDA-MB-231

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

DNA DNA quantification Human HeLa

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

DNA DNA quantification Human BMDM

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