shRNA gene silencing Human SiHa

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Monoclonal Mouse Anti-Human Ki-67 Antigen (Dako Omnis) Clone MIB-1 Product

Get tips on using Monoclonal Mouse Anti-Human Ki-67 Antigen (Dako Omnis) Clone MIB-1 to perform Immunohistochemistry Human - Ki-67

Products Agilent Technologies Monoclonal Mouse Anti-Human Ki-67 Antigen (Dako Omnis) Clone MIB-1
RNA isolation / purification Cells - primary human primary mammary adipose derived stem cells Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary human primary mammary adipose derived stem cells
hMSC Human Mesenchymal Stem Cell Chondrogenic Differentiation Basal Medium Product

Get tips on using hMSC Human Mesenchymal Stem Cell Chondrogenic Differentiation Basal Medium to perform Stem cell Differentiation media mPericytes differentiation into Chondrogenic cells

Products Lonza hMSC Human Mesenchymal Stem Cell Chondrogenic Differentiation Basal Medium
DNA methylation profiling Whole genome profiling - human peripheral blood mononuclear cells Experiment

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling human peripheral blood mononuclear cells
DNA methylation profiling Whole genome profiling - DU145, PC3 human prostate cancer Experiment

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling DU145, PC3 human prostate cancer
DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer Experiment

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling OVCAR-3 human ovarian cancer
Microarray Human - Precision cut lung slices Expression array Experiment

RNA Microarray Human Precision cut lung slices Expression array
DNA quantification Human - Colorectal aenocarenoma (SW48) - paraffin embeded Experiment

DNA DNA quantification Human Colorectal aenocarenoma (SW48) - paraffin embeded
Protein isolation Tissue - Human tissue C-MFPE samples Experiment

Proteins Protein isolation Tissue Human tissue C-MFPE samples
DNA Damage Assay Human bronchial epithelial cells (hBE) - Experiment

Cellular assays DNA Damage Assay Human bronchial epithelial cells (hBE)

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