Protein isolation

Get tips on using pcDNA™3.1D/V5-His TOPO®-hsEH to perform Protein Expression Eukaryotic cells - HEK293 hsEH

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using pLEXSY-hyg-2-hAm to perform Protein Expression Eukaryotic cells - Iranian lizard Leishmania cells recombinant human amelogenin

Get tips on using pPZP-RCS2-ocs-nptII to perform Protein Expression Prokaryotic cells - A. tumefaciens α-amylase, amylopullulanase, and glucoamylase

Get tips on using MicroVue YKL-40 EIA to perform ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Get tips on using pYT379-CDK8-CycC-10xHis complex to perform Protein Expression Eukaryotic cells - S. frugiperda CDK8-CycC-10xHis complex

Get tips on using Expi293™ Expression System Kit to perform Protein expression and purification Mammalian cells - HEK 293 Mt-PA

Get tips on using pET30 Ek/LIC-Colα3(IV) NC1 to perform Protein Expression Prokaryotic cells - E. coli Colα3(IV) NC1

Get tips on using pPICZα A, B, & C Pichia Vectors to perform Protein expression and purification Yeast - Pichia pastoris EDIII-D1