Immunohistochemistry Collagen Type I Goat Mouse

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - RAW264.7

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HeLa

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - Neuro 2a

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HK-2

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - CAKI-1

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HEK 293

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - MDA-MB-231

Get tips on using MUC2 (MRQ-18) Mouse Monoclonal Antibody to perform Immunohistochemistry Human - Muc-2