Immunohistochemistry Anti-mouse IgG Donkey Mouse

Get tips on using Acetyl-Histone H3 (Lys27) Antibody #4353 to perform ChIP Anti-bodies H3K27ac

Get tips on using TFIIB Antibody (D-3): sc-271736 to perform ChIP Anti-bodies TFIIB

Get tips on using STAT5 beta Monoclonal Antibody (ST5b-10G1) to perform ChIP Anti-bodies Stat5b

Get tips on using Stat5b Antibody (C-8): sc-377069 to perform ChIP Anti-bodies Stat5b

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using CD52 Monoclonal Antibody (CF1D12), PE to perform Flow cytometry Anti-bodies Human - CD52

Get tips on using CD70 Monoclonal Antibody (BU69), FITC to perform Flow cytometry Anti-bodies Human - CD70

Get tips on using GATA-3 Antibody (HG3-31): sc-268 to perform ChIP Anti-bodies GATA3

Get tips on using CD126 antibody | B-R6 to perform Flow cytometry Anti-bodies Human - CD126/IL-6Ralpha

Get tips on using Epithelial Antigen, Ber-EP4, Unconjugated to perform Flow cytometry Anti-bodies Human - CD326/EpCAM