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Bovine Brain Endothelial Cell Growth Medium Product

Get tips on using Bovine Brain Endothelial Cell Growth Medium to perform Mammalian cell culture media BBMVEC

Products Cell Applications Inc Bovine Brain Endothelial Cell Growth Medium

Cell Lysis Buffer (10X) Product

Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Tissue - Mouse lung tissue

Products Cell Signaling Technology Cell Lysis Buffer (10X)

Naive Pan T Cell Isolation Kit, human Product

Get tips on using Naive Pan T Cell Isolation Kit, human to perform Cell Isolation Naive Pan T cell

Products Miltenyibiotec Naive Pan T Cell Isolation Kit, human

cDNA synthesis Cell lines Experiment

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

RNA cDNA synthesis Cell lines

MEGM-Mammary-Epithelial-Cell-Growth-Medium Product

Get tips on using MEGM-Mammary-Epithelial-Cell-Growth-Medium to perform Mammalian cell culture media MCF-10A

Products Lonza MEGM-Mammary-Epithelial-Cell-Growth-Medium

RNA isolation / purification Cells - primary porcine primary airway epithelial cell Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary porcine primary airway epithelial cell

Double-negative T Cell Isolation Kit, human Product

Get tips on using Double-negative T Cell Isolation Kit, human to perform Cell Isolation Double-negative T Cell Isolation

Products Miltenyibiotec Double-negative T Cell Isolation Kit, human

Cell line authentication Human iPSC cells derived from human dermal fibroblasts Experiment

Cellular assays Cell line authentication Human iPSC cells derived from human dermal fibroblasts

MagniSort™ Human T cell Enrichment Kit Product

Get tips on using MagniSort™ Human T cell Enrichment Kit to perform Cell Isolation Human T cells

Products Thermo Fisher Scientific MagniSort™ Human T cell Enrichment Kit

EasySep™ Human T Cell Enrichment Kit Product

Get tips on using EasySep™ Human T Cell Enrichment Kit to perform Cell Isolation Human T cells

Products STEMCELL technologies EasySep™ Human T Cell Enrichment Kit

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