protein-isolation-tissue-mouse-heart

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Tissue - Human aortic endothelial cells

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using RIPA Lysis Buffer (ProteinSimple) to perform Protein isolation Tissue - Mouse aorta

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse prostate tissue

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse liver tissue

Get tips on using CelLytic™ NuCLEAR™ Extraction Kit to perform Protein isolation Tissue - Mouse cardiac tissue

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse lung tissue