Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Cells - primary mouse ventricles
Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Cells - primary mouse cardiomyocytes
DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.
DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
Get tips on using EZ1 RNA Tissue Mini Kit (48) to perform RNA isolation / purification Tissue - Human FFPE tissue
Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Brain
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Thymus
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Spleen
Get tips on using AllPrep DNA/RNA FFPE Kit to perform RNA isolation / purification Tissue - Mouse Prostate
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Ovarian
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