Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 to perform ChIP Rat - Mesenteric arteries
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine pulmonary artery endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine coronary artery endothelial cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine monocyte derived macrophages
Get tips on using ON-TARGETplus Rat Fyn siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Fyn
Get tips on using siGENOME Rat Gata1 (25172) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - C6 (rat glioma) Gata1
Get tips on using Mouse/Rat CD34 Antibody to perform Immunohistochemistry Mouse - CD34
Get tips on using Mouse/Rat TrkC Antibody to perform Immunohistochemistry Mouse - TrkC
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