RNA isolation / purification Cells primary

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SV 96 Total RNA Isolation System Product

Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell

Products Promega SV 96 Total RNA Isolation System

GeneJET RNA Purification Kit Product

Get tips on using GeneJET RNA Purification Kit to perform RNA isolation / purification Cells - primary human renal artery smooth muscle cells

Products Thermo Fisher Scientific GeneJET RNA Purification Kit

RNA isolation / purification Cells - immortalized EBL (embryonic lung cell) Experiment

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

RNA RNA isolation / purification Cells immortalized EBL (embryonic lung cell)

TRIzol™ Plus RNA Purification Kit Product

Get tips on using TRIzol™ Plus RNA Purification Kit to perform RNA isolation / purification Cells - primary rabbit aortic endothelial cells

Products Thermo Fisher Scientific TRIzol™ Plus RNA Purification Kit

Absolutely Total RNA Purification Kits Product

Get tips on using Absolutely Total RNA Purification Kits to perform RNA isolation / purification Cells - primary rat cardiac fibroblasts

Products Agilent Technologies Absolutely Total RNA Purification Kits

RNAqueous®-Micro Total RNA Isolation Kit Product

Get tips on using RNAqueous®-Micro Total RNA Isolation Kit to perform RNA isolation / purification Cells - primary human epithelial cells

Products Thermo Fisher Scientific RNAqueous®-Micro Total RNA Isolation Kit

High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human melanocytes

Products Roche Lifesciences High Pure RNA Isolation Kit

High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Products Roche Lifesciences High Pure RNA Isolation Kit

MagNA Pure Compact RNA Isolation Kit Product

Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Cells - primary porcine tracheal epithelial cells

Products Roche Lifesciences MagNA Pure Compact RNA Isolation Kit

SV 96 Total RNA Isolation System Product

Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - primary human airway epithelial cells

Products Promega SV 96 Total RNA Isolation System

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