Site Directed Mutagenesis (SDM) Human Point mutation SKOV3

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - SKOV3

Get tips on using Cell-APOPercentage™ Apoptosis Assay to perform Apoptosis assay cell type - SKOV3

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - SKOV3 AGR2

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - SKOV3

Get tips on using Cytochrome c Releasing Apoptosis Assay Kit to perform Apoptosis assay cell type - SKOV3

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - SKOV3 H19

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - SKOV3 PEG1

Get tips on using 24-Well Cell Invasion Assays, Basement Membrane to perform Cell migration / Invasion cell type - Skov3