CRISPR Mouse Deletion RAW 264.7

Get tips on using GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit to perform CRISPR Human - Activation ERBB2

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Repression Mstn

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Repression CCR-5

Get tips on using pLKO5.sgRNA.EFS.GFP to perform CRISPR Mouse - Activation Neuro-2a Smn1

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - Deletion C2C12 DMD

Get tips on using QuikChange Lightning Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Mouse - Deletion L929 ICP6

Get tips on using pcDNA-mC/EBPb to perform CRISPR Mouse - Activation 3T3-L1 C/EBPβ

Get tips on using lenti sgRNA(MS2)_zeo backbone to perform CRISPR Mouse - Activation C2C12 FST