Site Directed Mutagenesis (SDM) Human Deletion HepG2

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Human Genome CGH Microarray Kit 244A to perform Microarray Comperative genomic hybridization - Human HepG2

Get tips on using Accell Human VDAC1 (7416) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Human - HepG2 VADC1

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Human - HepG2

Get tips on using Silencer select_FGA siRNA to perform siRNA / miRNA gene silencing Human - HepG2 FGA

Get tips on using Silencer select_FGB siRNA to perform siRNA / miRNA gene silencing Human - HepG2 FGB

Get tips on using Silencer select_FGG siRNA to perform siRNA / miRNA gene silencing Human - HepG2 FGG

Get tips on using siRNA(h) ATG5 to perform siRNA / miRNA gene silencing Human - HepG2 ATG5

Get tips on using LowCell# ChIP kit protein A x16 to perform ChIP Human - HepG2