crispr-mouse-deletion-b117p-dck

Get tips on using pLV hUbC-dCas9-T2A-GFP to perform CRISPR Human - Repression HS2

Get tips on using pHR-SFFV-dCas9-BFP-KRAB to perform CRISPR Human - Repression CXCR4

Get tips on using pHR-SFFV-dCas9-BFP-KRAB to perform CRISPR Human - Repression lncRNA PVT1

Get tips on using pHR-SFFV-dCas9-BFP-KRAB to perform CRISPR Human - Repression lncRNA PVT1

Get tips on using pHR-SFFV-KRAB-dCas9-P2A-mCherry to perform CRISPR Human - Repression MYC

Get tips on using pHR-SFFV-KRAB-dCas9-P2A-mCherry to perform CRISPR Human - Repression GATA1

Get tips on using pAC154-dual-dCas9VP160-sgExpression to perform CRISPR Human - Activation HIV-1 5′ LTR

Get tips on using Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) to perform Flow cytometry Anti-bodies Mouse - CD16/CD32

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Mouse Reg1 Antibody to perform Immunohistochemistry Mouse - Reg1