The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis HUVEC
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells HUVEC
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells HUVEC
Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - HUVEC
Get tips on using Thiazolyl Blue Tetrazolium Bromide to perform Cell cytotoxicity / Proliferation assay cell type - HUVEC
Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HUVEC
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - HUVEC
Get tips on using RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay to perform Necrosis HUVEC
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