rna-isolation-purification-cells-immortalized-saos-2

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miRCURY RNA Isolation Kit Product

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized SK-BR-3

Products Exiqon miRCURY RNA Isolation Kit

Protein isolation Mammalian cells - SK-N-BE(2)-C Experiment

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Mammalian cells SK-N-BE(2)-C

SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Products Promega SV Total RNA Isolation System

SV Total RNA Isolation System Product

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized MCF-7

Products Promega SV Total RNA Isolation System

High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MCF 10A

Products Roche Lifesciences High Pure RNA Isolation Kit

High Pure RNA Isolation Kit Product

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized K-562

Products Roche Lifesciences High Pure RNA Isolation Kit

PicoPure™ RNA Isolation Kit Product

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized A-172

Products Thermo Fisher Scientific PicoPure™ RNA Isolation Kit

SV 96 Total RNA Isolation System Product

Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized T84

Products Promega SV 96 Total RNA Isolation System

HiPurA™ Total RNA Miniprep Purification Kit Product

Get tips on using HiPurA™ Total RNA Miniprep Purification Kit to perform RNA isolation / purification Cells - immortalized HaCaT

Products HiMEDIA HiPurA™ Total RNA Miniprep Purification Kit

RNAqueous®-Micro Total RNA Isolation Kit Product

Get tips on using RNAqueous®-Micro Total RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized T47D

Products Thermo Fisher Scientific RNAqueous®-Micro Total RNA Isolation Kit

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