Microarray Gene expression arrays Mouse liver tissue

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Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Mouse Point mutation L929 SigmaR1 gene (σ1)

Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Liver

Products Thermo Fisher Scientific MagMAX™-96 Total RNA Isolation Kit

RNA siRNA / miRNA gene silencing Mouse Glomerular mesangial cells HIPK2 Polymer / Lipid delivery
DMEM/F-12 Product

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse liver organoids

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Get tips on using MessageAmp II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Rhesus monkey brain tissue Biotin

Products Thermo Fisher Scientific MessageAmp II aRNA Amplification Kit

Get tips on using Cy3 Mono-Reactive Dye Pack to perform Microarray RNA amplification & Labeling - Human brain tissue Cyanine 3

Products GE Healthcare Life Sciences Cy3 Mono-Reactive Dye Pack

Get tips on using MessageAmp II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Human brain tissue Cyanine 3

Products Thermo Fisher Scientific MessageAmp II aRNA Amplification Kit

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - mouse kidney tissue

Products Qiagen RNeasy Lipid Tissue Mini Kit

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Mouse liver

Products Thermo Fisher Scientific LC3B Antibody Kit for Autophagy

Get tips on using BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector to perform shRNA gene silencing Mouse - P19 Foxm1

Products Thermo Fisher Scientific BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector

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