Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using MagMAX™-96 Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Liver
Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse liver organoids
Get tips on using MessageAmp II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Rhesus monkey brain tissue Biotin
Get tips on using Cy3 Mono-Reactive Dye Pack to perform Microarray RNA amplification & Labeling - Human brain tissue Cyanine 3
Get tips on using MessageAmp II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Human brain tissue Cyanine 3
Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - mouse kidney tissue
Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Mouse liver
Get tips on using BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector to perform shRNA gene silencing Mouse - P19 Foxm1
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