ROS assay cell type

Get tips on using DCFDA / H2DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - L-02 human fetal hepatocyte

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using MarkerGene™ Live Cell Fluorescent Reactive Oxygen Species Detection Kit to perform ROS assay cell type - T47D

Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - mouse cardiomyocytes

Get tips on using Fluoro hROS to perform ROS assay cell type - MiaPaCa-2 pancreatic carcinoma

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - PC12

Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - T47D

Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - PC-3 human prostate adenocarcinoma

Get tips on using Fluoro H202 to perform ROS assay cell type - Capan-2, PANC-1 pancreatic carcinoma