Site Directed Mutagenesis (SDM) Rat Point mutation H9C2

Get tips on using Orai1 Rat siRNA Oligo Duplex (Locus ID 304496) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Orai1

Get tips on using Rat TGF beta 1 ELISA Kit (ab119558) to perform ELISA Rat - TGF-beta 1

Get tips on using Rat IL-1 beta ELISA Kit (ab100768) to perform ELISA Rat - IL-1 beta

Get tips on using Rat ICAM-1 PicoKine™ ELISA Kit to perform ELISA Rat - ICAM-1/CD54

Get tips on using Rat ICAM-1/CD54 Quantikine ELISA Kit to perform ELISA Rat - ICAM-1/CD54

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines H9C2

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Live / Dead assay mammalian cells - H9C2

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Rat C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Rat - C-Reactive Protein/CRP

Get tips on using PE Rat Anti-Mouse CD103 to perform Flow cytometry Anti-bodies Mouse - CD103