Site Directed Mutagenesis (SDM) Monkey Point mutation Vero

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Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Products Thermo Fisher Scientific Lipofectamine® 2000 Transfection Reagent

Get tips on using Gibco™ DMEM, high glucose, GlutaMAX™ Supplement to perform 3D Cell Culture Media Mouse small intestinal organoids

Products Thermo Fisher Scientific Gibco™ DMEM, high glucose, GlutaMAX™ Supplement

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary rat aortic smooth muscle cells

Products Thermo Fisher Scientific mirVana™ miRNA Isolation Kit, with phenol

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Rat aortic smooth muscle cells (rASMC)

Products Polyplus transfections jetPEI® DNA transfection, HTS application

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells

Products Thermo Fisher Scientific mirVana™ miRNA Isolation Kit, with phenol

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Rat primary vascular smooth muscle cells Biotin

Products Thermo Fisher Scientific FlashTag™ Biotin HSR RNA Labeling Kits

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human aortic smooth muscle cells (HOSMC)

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - rat aortic smooth muscle cells

Products Thermo Fisher Scientific LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven

Cellular assays Cell migration / Invasion cell type SH-SY5Y

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Cellular assays Cell migration / Invasion cell type HeLa

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