Protein quantification

Get tips on using BlueRAY Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using BLUelf Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using BlueAQUA Prestained Protein Ladder to perform Protein Ladder Prestained

Get tips on using BLUeye Prestained Protein Ladder to perform Protein Ladder Prestained

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Hoechst 33258 to perform DNA quantification Human - MDA-MB-231

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Human - MCF10A

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Mouse - EMT6

Get tips on using Agilent RNA 6000 Pico Kit to perform RNA quantification Fuorimetric