Live / Dead assay yeast

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - PANC-, BxPC-3 human pancreas

Get tips on using Beta-Lactamase Activity Assay Kit to perform Reporter gene assay β-lactamase substrates - Burkholderia cepacia complex

Get tips on using In Vitro ROS/RNS Assay to perform ROS assay cell type - human umbelical vein endothelial cells (HUVEC)

Get tips on using MTT Cell Growth Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - oral squamous cell carcinoma

Get tips on using Cellular Senescence Flow Cytometry Assay to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Get tips on using Beta-Glo® Assay System to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Get tips on using TUNEL Assay Kit - BrdU-Red to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.