dna-quantification-human-hela

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary hematopoietic stem cells

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized Hepa-1c1c7

Get tips on using Anti-Beclin 1 antibody [2A4] (ab114071) to perform Autophagy assay cell type - Hepatocytes

Get tips on using Anti-Beclin 1 antibody [2A4] (ab114071) to perform Autophagy assay cell type - Hepatocytes

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Hepatocytes

Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Mouse - Hepa-1

Get tips on using EZ-Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Hepa-1

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - Cancer cell lines Liver cancer cell lines Hepato cellular carcenoma (SMMC-7721, Huh7 & HepG2))

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - PLHC-1 poeciliopsis lucida hepatocellular carcinoma

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.