crispr-mouse-activation-3t3-l1-c-ebp

Get tips on using ON-TARGETplus Mouse Stat3 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 STAT3

Get tips on using ON-TARGETplus Mouse Prkaa1 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Prkaa1

Get tips on using Purified Mouse Anti-Nucleoporin p62 to perform Western blot p62/SQSTM1 - Mouse IgG2b Human -NA-

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines 3T3

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - NIH-3T3

Get tips on using ScriptSeq Complete Kit (Human/Mouse/Rat) to perform RNA sequencing Mouse - J774

Get tips on using Mouse ICAM1 ELISA Kit (CD54) (ab100688) to perform ELISA Mouse - ICAM-1/CD54

Get tips on using Mouse Gene Expression v2 4x44K Microarray Kit to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.