Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using Omniscript RT Kit (200) to perform cDNA synthesis Cell lines
Get tips on using Sensiscript RT Kit (200) to perform cDNA synthesis Cell lines
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized 293T
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized VCaP
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized U87
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized T98G
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Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized LNCaP
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