rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human preadipocytes

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human osteoblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human chondrocytes

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human keratinocytes

Get tips on using ISOGEN to perform RNA isolation / purification Cells - primary human epidermal melanocytes

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human lung fibroblasts (HLF)

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary canine peripheral blood mononuclear cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood mononuclear cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary porcine coronary artery endothelial cells